Barw Barw Medical Journal 2960-1959 2024 06 20 Variation in Parameters of Complete Blood Count Using Ethylene Diamine Tetra Acetic Acid (EDTA), Sodium Citrate and Lithium Heparin: A Cross-Sectional Study 10.58742/bmj.v2i2.90 eng Sharaza Qadir Omer Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Kurdistan, Iraq. sharaza.omer@gmail.com Rawezh Q. Salih Department of Biology, College of Science, University of Sulaimani, Sulaymaniyah, Kurdistan, Iraq. rawizh.mohammed94@gmail.com Shvan Luqman Ezzat Department of Biology, College of Science, University of Sulaimani, Sulaymaniyah, Kurdistan, Iraq. shvan.ezzat@gmail.com Dahat A. Hussein Department of Biology, College of Science, University of Sulaimani, Sulaymaniyah, Kurdistan, Iraq. dahat.hussein@gmail.com Ayman M. Mustafa Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Kurdistan, Iraq. aymanmajid@gmail.com Aga Khdir Hamad Medical Laboratory Science, College of Health Science, University of Duhok, Duhok, Kurdistan, Iraq. aga.hamad@gmail.com Hawnaz Salih Abdullah Department of Biology, College of Science, University of Sulaimani, Sulaymaniyah, Kurdistan, Iraq. hawnazsalh364@gmail.com Bayan Aziz Saeed Department of Biology, College of Science, University of Sulaimani, Sulaymaniyah, Kurdistan, Iraq. bayan.saeed@gmail.com Shahid Salah Rashid Department of Biology, College of Science, Koya University, Erbil, Kurdistan, Iraq . shahid@gmail.com Sangar Jum’a Department of Chemistry, College of Science, University of Sulaimani, Sulaymaniyah, Kurdistan, Iraq. sangar12@gmail.com Mohammed Hassan Sofi Department of Biology, College of Science, University of Sulaimani, Sulaymaniyah, Kurdistan, Iraq. Mohammed.Sofi@gmail.com 2024 04 07 Introduction The primary anticoagulants utilized in hematology are ethylene diamine tetra acetic acid (EDTA), lithium heparin, and citrate. Although various studies have investigated their impact on complete blood count (CBC) in various animals, limited data are available concerning humans. This study explores the differences in CBC parameters when using these anticoagulants. Methods This study was a cross-sectional study comprising 250 participants who underwent CBC tests with different anticoagulants. Blood samples were examined using the Medonic M51. Statistical analyses, including one-way ANOVA, intra-class correlation coefficient, and concordance correlation coefficient, were employed as applicable, with significance level set at p-values <0.05. Results One hundred eight participants (43.2%) were male, and 142 (56.8%) were female, with a mean age of 40.88±17.06 years ranging from 7 to 91 years.  Comparing K2EDTA with lithium heparin, comparable values were found in 14 out of 23(60.87%) CBC parameters, while citrate showed similar results in 13(56.52%) parameters. Using K2EDTA as the standard, citrate showed perfect or substantial agreement in assessing 9 out of 23 CBC parameters (39.13%). Similarly, Lithium heparin showed perfect or substantial agreement in determining 9 out of 23(39.13%) parameters. Compared to K2EDTA, lithium heparin exhibited high precision and accuracy in estimating 13 out of 23(56.52%) CBC parameters. In contrast, citrate was accurate in 9 out of 23(39.13%) parameters. Conclusion Using citrate instead of K2EDTA for CBC estimation may yield inaccurate outcomes, whereas lithium heparin could serve as an alternative anticoagulant, requiring careful monitoring.