Barw Barw Medical Journal 2960-1959 2025 11 17 Impact of Common Anticoagulants on Complete Blood Count Parameters Among Humans 7 13 10.58742/bmj.v4i1.212 eng Rawezh Q. Salih Kscien Organization for Scientific Research (Middle East Office), Hamdi Street, Sulaymaniyah, Iraq. Rawezh.mohammed@univsul.edu.iq Dahat A. Hussein Department of Laboratory, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. dahat.abdulla18@gmail.com Sharaza Q. Omer Department of Laboratory, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. sharaza.omer@gmail.com Shvan L. Ezzat Department of Laboratory, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. shvan.ezzat@gmail.com Ayman M. Mustafa Department of Scientific Affairs, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. aymanmajid75@gmail.com Hawnaz S. Abdullah Department of Laboratory, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. hawnazsalh364@gmail.com Hidayat A. Yaseen Department of Laboratory, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. hidayat45@gmail.com Mohammed T. Taha Department of Laboratory, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. mohammed.taha@gmail.com 2025 09 05 Introduction Among the most frequently used anticoagulants in hematological testing are tetra-acetic acid (EDTA), sodium citrate, and sodium heparin. However, there is a noticeable gap in literature concerning the effects of these anticoagulants on hematological parameters specifically in humans. This study aims to assess the effectiveness of EDTA, sodium citrate, and sodium heparin for conducting complete blood count (CBC). Methods This cross-sectional study conducted at Smart Health Tower from January to April 2024 involved 250 participants who underwent CBC using K2EDTA, sodium citrate, and sodium heparin. The acquired data were analyzed using SPSS, with a significance level of p < 0.05, employing Intra-class correlation coefficient and one-way ANOVA to assess consistency and agreement among anticoagulants. Results A total of 250 participants, with 138(55.2%) males and 112(44.8%) females, underwent CBC testing with di potassium EDTA(K2EDTA), sodium citrate, and sodium heparin. Comparing K2EDTA with sodium heparin showed comparable values in 14 out of 23(60.87%) CBC parameters. Using K2EDTA as the standard, citrate showed perfect or substantial agreement in assessing 8 out of 23 CBC parameters (34.78%). Regarding the comparison of anticoagulants to K2EDTA to determine their agreement levels while sodium heparin was accurate and precise in 13(56.52%) parameters. Conclusion Citrate was found to be a less reliable anticoagulant for CBC estimation compared to K2EDTA, potentially leading to inaccurate readings. On the other hand, sodium heparin showed comparable performance to K2EDTA, making it a suitable alternative under specific conditions.