Barw Barw Medical Journal 2960-1959 2024 11 25 Blood Cross Matching Without Anti-Human Globulin (AHG) and Bovine Serum: A New Interest for an Old Idea 10.58742/bmj.v2i4.136 eng Suhaib H. Kakamad College of Medicine, Department of Immunology and Hematology, Kurdistan University of Medical Science, Sanandaj, Iran. Ohawar027@gmail.com Mohieddin Barzegar College of Paramedical, Department of Laboratory Science, Kurdistan University of Medical Science, Sanandaj, Iran. Ohawar027@gmail.com Mohammad Reza Rahmani College of Medicine, Department of Immunology and Hematology, Kurdistan University of Medical Science, Sanandaj, Iran. Suhaibhusein43@gmail.com 2024 10 18 Introduction Transfusion medicine promotes the safety of blood transfusions by rigorously testing to eliminate risks of infection and hemolytic. The efficacy (to correct and identify antibodies) of a modified cross-matching method that excludes the use of anti-human globulin (AHG) bovine serum with that of the immediate spin crossmatch was assessed. Material and Methods This multi-center study was performed at two medical centers in Iran and Iraq. Over seven years, consecutive participants received two different blood cross-matching methods: one with AHG and bovine serum and another without it. Results The study included 31240 participants. About 18526 (59.3%) were males and 12714 (40.7%) were females. The ages of participants ranged from 23 to 57 years, with the average age of 39 years. Only 45 (0.14%) participants blood cross-match incompatible in both spin cross-match and cross-match without AHG, bovine serum the same result. The study found that all detected antibodies correlated with potential blood incompatibility, and there remained 100% safety between the two testing methods. Conclusion Omitting AHG and bovine serum in cross-matching might be safe. At least it can be used in emergency situations and in resource-limited settings.